• Chapter 1. Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green Fluorescent Protein. David W. Galbraith, Jen Sheen, Georgina M. Lambert, and Robert J. Grebenok.
• Chapter 2. Functional effects of structural changes in photosystem II as measured by chlorophyll fluorescence kinetics. Wim F.J. Vermaas.
• Chapter 3. Determination of protein isoprenylation in vitro and in vivo. Jian-Kang Zhu, Ray A. Bressan, and Paul M. Hasegawa.
• Chapter 4. Extraction and assay of protein from single plant cells. William H. Outlaw Jr.
• Chapter 5. Photoaffinity labeling and strategies for plasma membrane protein purification. Joachim Feldwisch, Andreas Vente, Narciso Campos, Rolf Zettl, and Klaus Palme.
• Chapter 6. Cell Cycle Synchronization, Chromosome Isolation, and Flow-Sorting in Plants. Sergio Lucretti and Jaroslav Dolezel.
• Chapter 7. Principles and Applications of Recombinant Antibody Phage Display Technology to Plant Biology. William L. Crosby and Peter Schorr.
• Chapter 8. Isolation and Characterization of Plant Nuclei. Tom J. Guilfoyle
• Chapter 9. Isolation of Nuclei Suitable for in vitro Transcriptional Studies. John C. Cushman.
• Chapter 10. Analysis of the H+-ATPase and Other Proteins of the Arabidopsis Plasma Membrane. G. Eric Schaller and Natalie D. DeWitt.
• Chapter 11. Specific examples in subcellular fractionation: Isolation and reconstitution of the vacuolar H+-ATPase. John M. Ward and Heven Sze.
• Chapter 12. Isolation and Fractionation of Plant Mitochondria and Chloroplasts: Specific Examples. Jose Manuel Gualberto, Hirokazu Handa and Jean Michel Grienenberger.
• Chapter 13. Isolating the Plant Mitotic Apparatus: A Procedure for Isolating Spindles from the Diatom Cylindrotheca fusiformis. Harrison Wein, Barbara Brady, and W. Zacheus Cande.
• Chapter 14. Chromoplasts. C. A. Price, Noureddine Hadjeb, Lee A. Newman, and Ellen M. Reardon.
• Chapter 15. Methods for isolation and analysis of polyribosomes. Eric Davies and Shunnosuke Abe.
• Chapter 16. Methods for isolation and analysis of the cytoskeleton. Shunnosuke Abe and Eric Davies.
• Chapter 17. Isolation and characterization of plasmodesmata. Bernard L. Epel, Bella Kuchuck, Guy Kotlizky, Shomrat Shurtz, Michael Erlanger, and Avital Yahalom.
• Chapter 18. Characterization and isolation of the chloroplast protein import machinery. Karin Waegemann and Jurgen Soll.
• Chapter 19. Macromolecular movement into mitochondria. Elzbieta Glaser, Carina Knorpp, Marie Hugosson and Erik von Stedingk.
• Chapter 20. Targeting of Proteins to the Nucleus. James C. Carrington.
• Chapter 21. Import into the Endoplasmic Reticulum. Aldo Ceriotti, Emanuela Pedrazzini, Marcella de Silvestris and Alessandro Vitale.
• Chapter 22. Protein-Protein Interactions in the Endoplasmic Reticulum. Jeffrey W. Gillikin, Elizabeth P. B. Fontes and Rebecca S. Boston.
• Chapter 23. Assaying Proteins for Molecular Chaperone Activity. Garrett J. Lee.
• Chapter 24. The use of protoplasts to study protein synthesis and transport by the plant endomembrane system. Jurgen Denecke and Alessandro Vitale.
• Chapter 25. Assessment of Translational Regulation by Run-Off Translation of Polysomes in vitro. Michael E. Vayda.
• Chapter 26. Electroporation of Plant Protoplasts and Tissues. George W. Bates.
• Chapter 27. Particle Bombardment. Paul Christou.
• Chapter 28. Preparation and Transformation of Monocot Protoplasts. C. Maas, C. Reichel, J. Schell and H.-H. Steinbiss.
• Chapter 29. Tobacco Protoplast Transformation and Use for Functional Analysis of Newly Isolated Genes and Gene Constructs. Regina Fischer and Rndiger Hain.
• Chapter 30. Novel inducible/repressible gene systems. Christiane Gatz.
• Chapter 31. Reporter Genes. Clemens Suter-Crazzolara, Manfred Klemm and Bernd Reiss.
• Chapter 32. Cell-Specific Ablation in Plants. Mary K. Thorsness and June B. Nasrallah.
• Chapter 33. Ribozymes. Peter Steinecke and Peter H. Schreier.
• Chapter 34. Expression of plant proteins in baculoviral and bacterial systems. Reinhard Kunze, Heidi Fubwinkel and Siegfried Feldmar.
• Chapter 35. Expression and localization of plant membrane proteins in Saccharomyces. Ramon Serrano and Jose-Manuel Villalba.
• Chapter 36. Synthesis of plant proteins in heterologous systems: Xenopus laevis oocytes. Gad Galili, Yoram Altschuler and Aldo Ceriotti.
• Chapter 37. Heterologous Expression of Higher Plant Transport Proteins and Repression of Endogenous Ion Currents in Xenopus Oocytes. Julian I. Schroeder.

Editors: David Galbraith, Don Bourque, and Hans Bohnert

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